ICC testing of human HeLa cells treated with sodium butyrate using recombinant H3K14ac antibody (red). Actin filaments have been labeled with fluorescein phalloidin (green).

ChIP performed on HeLa cells using the recombinant H3K14ac antibody (5ug). Real-time PCR was performed using primers specific to the gene indicated.

The recombinant H3K14ac antibody specifically reacts to Histone H3 acetylated at Lysine 14 (K14ac). No cross reactivity with acetylated Lysine 4 (K4ac), 9 (K9ac), 18 (K18ac), 23 (K23ac), 27 (K27ac), 36 (K36ac), or 79 (K79ac) in Histone H3.

The binding specificity of recombinant H3K14ac antibody to Histone H3 acetylated at Lysine 14 (K14ac) is not affected by the modification of neighboring amino acids.